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Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
28/01/2016 |
Data da última atualização: |
26/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 3 |
Autoria: |
MÜLLER, L. S.; SILVEIRA, G. D. da; PRÁ, V. D.; LAMEIRA, O.; VIANA, C.; CARVALHO, L. M. de. |
Afiliação: |
Larissa Sabo Müller, GRADUANDA UFSM; Géssica Domingos da Silveira, UFSM; Valéria Dal Prá, GRADUANDA UFSM; OSMAR ALVES LAMEIRA, CPATU; Carine Viana, GRADUANDA UFSM; Leandro Machado de Carvalho, UFSM. |
Título: |
Investigation of phenolic antioxidants as chemical markers in extracts of Connarus perrottetii var. Angustifolius Radlk by capillary zone electrophoresis. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Journal of Liquid Chromatography & Related Technologies, v. 39, n. 1, p. 13-20, 2016. |
DOI: |
10.1080/10826076.2015.1115768 |
Idioma: |
Inglês |
Conteúdo: |
Plant roots, leaves, barks, seeds berries, or flowers can be used to promote health and treat diseases and also have compounds that provide information about the best quality of raw materials. A medicinal plant native to the Amazonia region (Brazil) was investigated in this work. For this purpose, a new analytical approach was developed by capillary zone electrophoresis (CZE) with UV detection for the separation of 14 phenolic compounds extracted from the plant extracts. The method enabled simultaneous determination of 3-acetylcoumarin, resveratrol, 6-hydroxycoumarin, catechin, rutin, ferulic acid, quercitrin, kaempferol, fisetin, myricetin, quercetin, caffeic acid, gallic acid, and 4-hydroxycinnamic acid using borate buffer (20 mM, pH 9.2) containing 15% methanol (v/v) as working electrolyte, separation potential of ?20 kV, separation temperature of 25°C and hydrodynamic injection by gravity (20 cm for 60 s). The developed method was validated, obtaining repeatability and inter-day precision values lower than 7% and 6%, respectively. Recovery was performed and ranged from 84% to 118%. The method permitted the quantification of catechin and rutin in Connarus perrottetii var. angustifolius aqueous infusions, ethanolic extracts, and butanolic extracts (both obtained after maceration). The radical scavenging activity of the extracts toward free radicals is also described. |
Palavras-Chave: |
Marcador químico. |
Thesagro: |
Antioxidante; Planta Medicinal. |
Thesaurus NAL: |
Amazonia. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 02203naa a2200241 a 4500 001 2035420 005 2022-05-26 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1080/10826076.2015.1115768$2DOI 100 1 $aMÜLLER, L. S. 245 $aInvestigation of phenolic antioxidants as chemical markers in extracts of Connarus perrottetii var. Angustifolius Radlk by capillary zone electrophoresis.$h[electronic resource] 260 $c2016 520 $aPlant roots, leaves, barks, seeds berries, or flowers can be used to promote health and treat diseases and also have compounds that provide information about the best quality of raw materials. A medicinal plant native to the Amazonia region (Brazil) was investigated in this work. For this purpose, a new analytical approach was developed by capillary zone electrophoresis (CZE) with UV detection for the separation of 14 phenolic compounds extracted from the plant extracts. The method enabled simultaneous determination of 3-acetylcoumarin, resveratrol, 6-hydroxycoumarin, catechin, rutin, ferulic acid, quercitrin, kaempferol, fisetin, myricetin, quercetin, caffeic acid, gallic acid, and 4-hydroxycinnamic acid using borate buffer (20 mM, pH 9.2) containing 15% methanol (v/v) as working electrolyte, separation potential of ?20 kV, separation temperature of 25°C and hydrodynamic injection by gravity (20 cm for 60 s). The developed method was validated, obtaining repeatability and inter-day precision values lower than 7% and 6%, respectively. Recovery was performed and ranged from 84% to 118%. The method permitted the quantification of catechin and rutin in Connarus perrottetii var. angustifolius aqueous infusions, ethanolic extracts, and butanolic extracts (both obtained after maceration). The radical scavenging activity of the extracts toward free radicals is also described. 650 $aAmazonia 650 $aAntioxidante 650 $aPlanta Medicinal 653 $aMarcador químico 700 1 $aSILVEIRA, G. D. da 700 1 $aPRÁ, V. D. 700 1 $aLAMEIRA, O. 700 1 $aVIANA, C. 700 1 $aCARVALHO, L. M. de 773 $tJournal of Liquid Chromatography & Related Technologies$gv. 39, n. 1, p. 13-20, 2016.
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